A preliminary revision of the palynological collection of Professor Blanka Pacltová was carried out considering samples from the middle Cenomanian of the Peruc-Korycany Formation, the basal most member of the Bohemian Cretaceous Basin (the Czech Republic). This collection is mainly composed of slides with palynological residues for light microscope study, which were mounted in the 1960s and 1970s. This work presents an evaluation of the state of preservation of this collection, taking into account the presence of ancient angiosperm pollen types. High percentage of preparations is affected by degradation of glycerine jelly and their remounting is necessary. The present study additionally suggests a methodology for curation of this collection with the objective of long-term preservation.